Dynamic full-field optical coherence tomography module adapted to commercial microscopes allows longitudinal in vitro cell culture study - ANR - Agence nationale de la recherche Accéder directement au contenu
Article Dans Une Revue Communications Biology Année : 2023

Dynamic full-field optical coherence tomography module adapted to commercial microscopes allows longitudinal in vitro cell culture study

Tual Monfort
Salvatore Azzollini
Marilou Clémençon
Serge Picaud
Olivier Goureau
Sacha Reichman
Olivier Thouvenin
  • Fonction : Auteur
Kate Grieve

Résumé

Abstract Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as a label-free imaging tool, capable of resolving cell types and organelles within 3D live samples, whilst monitoring their activity at tens of milliseconds resolution. Here, a D-FFOCT module design is presented which can be coupled to a commercial microscope with a stage top incubator, allowing non-invasive label-free longitudinal imaging over periods of minutes to weeks on the same sample. Long term volumetric imaging on human induced pluripotent stem cell-derived retinal organoids is demonstrated, highlighting tissue and cell organization processes such as rosette formation and mitosis as well as cell shape and motility. Imaging on retinal explants highlights single 3D cone and rod structures. An optimal workflow for data acquisition, postprocessing and saving is demonstrated, resulting in a time gain factor of 10 compared to prior state of the art. Finally, a method to increase D-FFOCT signal-to-noise ratio is demonstrated, allowing rapid organoid screening.
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Dates et versions

hal-04287440 , version 1 (02-07-2024)

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Tual Monfort, Salvatore Azzollini, Jérémy Brogard, Marilou Clémençon, Amélie Slembrouck-Brec, et al.. Dynamic full-field optical coherence tomography module adapted to commercial microscopes allows longitudinal in vitro cell culture study. Communications Biology, 2023, 6 (1), pp.992. ⟨10.1038/s42003-023-05378-w⟩. ⟨hal-04287440⟩
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