Imaging on Plant Tissues and Organs : from tissue patterning to molecular labelling
Résumé
The plant histology and imaging platform aim to see the plant cell in all its states, locate in situ the main molecules of living things: proteins, nucleic acids, secondary metabolites.
On this platform, one can use classical histology experiments to visualize plants tissue structures using different coloration (toluidine blue, phlorogluvinol coloration) and molecular histology techniques to study molecules of interest. This can be achieved with markers such as GUS for the localisation of gene expression patterns or by using specific probes for RNAs, or antibodies for proteins. We can use whole-mount in situ or hybridation on sections tissue. For sections tissue, we cut fresh tissue included in agarose using vibratome or fixed tissue included in parraffin or resin using microtome. All the observation of the slides and the acquisition of the images is done with a zeiss axioscope microscope and analyzed with the zen software. This plateform is located in the PCV laboratory in building C2 of CEA directed by Christel CARLES, the scientific manager and Marie LE MASSON, the technical manager.
Domaines
Sciences du Vivant [q-bio]Origine | Fichiers produits par l'(les) auteur(s) |
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