Determination of Multimodal Isotopic Distributions: The Case of a (15)N Labeled Protein Produced into Hairy Roots. - ANR - Agence nationale de la recherche Accéder directement au contenu
Article Dans Une Revue Analytical Chemistry Année : 2015

Determination of Multimodal Isotopic Distributions: The Case of a (15)N Labeled Protein Produced into Hairy Roots.

Laurent Coquet
Julie Hardouin
Pascal Cosette

Résumé

Isotopic labeling is widely used in various fields like proteomics, metabolomics, fluxomics, as well as in NMR structural studies, but it requires an efficient determination of the isotopic enrichment. Mass spectrometry is the method of choice for such analysis. However, when complex expression systems like hairy roots are used for production, multiple populations of labeled proteins may be obtained. If the isotopic incorporation determination is actually well-known for unimodal distributions, the multimodal distributions have scarcely been investigated. Actually, only a few approaches allow the determination of the different labeled population proportions from multimodal distributions. Furthermore, they cannot be used when the number of the populations and their respective isotope ratios are unknown. The present study implements a new strategy to measure the 15N labeled populations inside a multimodal distribution knowing only the peptide sequence and peak intensities from mass spectrometry analyses. Noteworthy, it could be applied to other elements, like carbon and hydrogen, and extended to a larger range of biomolecules.
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Dates et versions

hal-01186525 , version 1 (25-08-2015)

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Romain Trouillard, Marie Hubert-Roux, Vincent Tognetti, Laure Guilhaudis, Carole Plasson, et al.. Determination of Multimodal Isotopic Distributions: The Case of a (15)N Labeled Protein Produced into Hairy Roots.. Analytical Chemistry, 2015, 87 (12), pp.5938-5946. ⟨10.1021/acs.analchem.5b01558⟩. ⟨hal-01186525⟩
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