Thermoresponsive gel embedding extracellular vesicles from adipose stromal cells improves the healing of colonic anastomoses following irradiation in rats - Institut Curie
Article Dans Une Revue Communications Biology Année : 2024

Thermoresponsive gel embedding extracellular vesicles from adipose stromal cells improves the healing of colonic anastomoses following irradiation in rats

Anna Sebbagh
Arthur Berger
  • Fonction : Auteur

Résumé

Anastomotic leak occurrence is a severe complication after colorectal surgery. Considering the difficulty of treating these leaks and their impact on patient care, there is a strong need for an efficient prevention strategy. We evaluated a combination of extracellular vesicles (EVs) from rat adiposederived stromal cells with a thermoresponsive gel, Pluronic® F127 (PF-127) to prevent anastomotic leaks. The pro-regenerative and immunomodulatory potencies of EVs are assessed in vitro. In vivo efficacy are assessed in rat with a colonic anastomosis model after irradiation. Endoscopic, anatomical and histological data show a consistent effect of EVs + gel on the healing of colonic anastomosis. These results are illustrated by a smaller anastomotic ulcer size, less fibrosis and less inflammatory infiltrations in the EVs + gel group. This multi-modal investigation is the first to point-out the translational potential of EVs combined with PF-127 for the healing of high-risk colorectal anastomosis.

The incidence of rectal cancer worldwide is 732,210 per year 1 . Neoadjuvant chemoradiotherapy supplemented by surgery with a low colorectal or colo-anal anastomosis is recommended in locally advanced disease 2 . Despite years of research, the incidence of anastomotic leak after rectal surgery remains high, ranging from 3 to 21% 3,4 and is associated with increased morbidity, mortality, and cancer recurrence 5 . Furthermore, anastomotic leaks are associated with an increased healthcare burden due to the higher costs of management and the use of resources for remedial care 6 . It is known that anastomotic leak etiology is multifactorial. Excluding technical failures, there is accumulating evidence pointing at anastomotic leak as the result of a complex, dynamic interplay of several factors and biological processes 7-9 . This includes host genetics, gut microbiome, inflammation, and the immune system 10 . The occurrence of anastomotic leak might also be linked to TNM stage and patient nutritional status 11 , along with treatment-related factors. Indeed, while preoperative chemoradiotherapy for rectal cancer improves local

Fichier principal
Vignette du fichier
Alric_et_al-2024-Communications_Biology (1).pdf (2.27 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

irsn-04877808 , version 1 (09-01-2025)

Licence

Identifiants

Citer

Hadrien Alric, Noelle Mathieu, Anna Sebbagh, Guillaume Peré, Christelle Demarquay, et al.. Thermoresponsive gel embedding extracellular vesicles from adipose stromal cells improves the healing of colonic anastomoses following irradiation in rats. Communications Biology, 2024, 7 (1), pp.1673. ⟨10.1038/s42003-024-07364-2⟩. ⟨irsn-04877808⟩
0 Consultations
0 Téléchargements

Altmetric

Partager

More