Restoration of mitochondria cristae in live microscopy images
Résumé
Mitochondria play an essential role in the life cycle of eukaryotic cells. However, we still don't know how their ultrastructure, like the cristae of the inner membrane, dynamically evolves to regulate these fundamental functions, in response to external conditions or during interaction with other cell components. Although high-resolution fluorescent microscopy coupled with recently developed innovative probes can reveal this structural organization, their long-term, fast and live 3D imaging remains challenging. To address this problem, we have developed a convolutional neural network to restore mitochondrial cristae in low spatial resolution microscopy images. Depending on the conditions of use indicated, our method works well on broad microscopy modalities (STED, Live-SR and LLSM). It is ultimately applied in the context of mitochondrial network dynamics during interaction with endo/lysosomes membranes.
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